Abstract

Single Cell and Subcellular Expression Profiling; Insight into Mechanisms Behind Asymmetric Cell Division

Mikael Kubista, Professor/Founder, TATAA Biocenter AB

Biological samples are composed of large number of cells of different types. When studying traditional samples containing many cells only the collective response of all the cells present is measured. However, the cells may respond differently and a small subpopulation of stem cell character may be critical. Today, samples can be disintegrated into individual cells and characterized phenotypically by the expression of key mRNA, microRNA or protein markers.Since its development in 2005 single cell profiling is rapidly becoming one of the most promising platforms for biological characterization of minority cell types offering great promise to elucidate mechanisms of cell differentiation. In the same year, we also the key markers for human embryonic stem cells.