Abstract

Microfluidic Multiplexing For Immune Cell Detection In Tumor Sections

Daniel Migliozzi, Student, EPFL

We developed a polymer-and-glass chip for fast delivery of reagents on tissue slides and fully automatic imaging by integration with an optical microscope, along with high-throughput image-processing for single cell mapping. As proof-of-concept analyses, we identified co-expression and co-localization patterns of biomarkers to classify the immune cells and their activation status in lung adenocarcinoma.