Abstract

On-Chip Liquid Biopsy: Progress in Isolation of Exosomes and Their RNA Sequencing For Prognosis of Prostate Cancer

Navneet Dogra, Researcher, IBM

Exosomes are an exciting target for “liquid biopsies”. However, isolation of exosomes and reproducible detection of their biomarkers remains an ongoing challenge. We have developed a microfluidic nanoscale DLD (Deterministic lateral displacement) device that brings capabilities with size based sorting of colloidal particles at the tens of nanometers scale (Wunsch et al. , Nature Nanotechnology 2016). I will present our most recent findings using RNA sequencing of cancer cell lines and patient samples.