Abstract

Delivering Functional Hepatocytes from Pluripotent Stem Cells

David Hay, Principal Investigator, MRC Centre for Regenerative Medicine, University of Edinburgh

The development of renewable human liver models, from genetically defined origins, is likely to be a game-changing addition to the field. However, for these models to have significant impact they must be stable in character and capable of manufacture at scale. For this reason, we have chosen to use pluripotent stem cells (PSCs). We have developed highly efficient, scalable, and reliable differentiation procedures for use with PSCs, which demonstrate very promising qualities. In collaboration with industry and academic laboratories, we provide proof of concept that stem cell derived hepatocytes are scalable and metabolise drugs. In addition, our stem cell based systems possess an intact innate immune system and are capable of predicting human drug-induced liver injury, in line with current gold standard human models. Our most recent advances will be discussed at the meeting.