Abstract

The Significant Function of EVs in Aging and Cancer

Hidetoshi Tahara, Deputy Executive Director, Industry-Academia Collaboration; Professor, Department of Cellular and Molecular Biology; Head, The Research Center for Drug development and Biomarker Discovery, Hiroshima University

Extracellular vesicles (EVs) are nano-sized vesicles and carry various cargo, including microRNAs, mRNAs and proteins, released into the extracellular environment and can be used for cell-to-cell communication in vivo. Recently we found that the secretion of EVs in senescent fibroblast cells is significantly higher than those in young cells, and interestingly senescence-derived EVs inhibit cell proliferation, colony formation and invasion of cancer cell lines. To understand the function of these EVs from senescence cells, we analyzed exRNA by using a next generation sequencer, Ion PGM. Interestingly, MicroRNA profiling of EVs is significantly altered between senescent cells and young cells. Several significant tumor suppressive microRNAs which contained in senescence derived EVs significantly inhibit tumor invasion and cell growth in breast and cervical cancer cell lines. We also analyze EVs RNA in cancer cell lines using NGS, and found specific microRNAs are altered in cancer cells. Surprisingly, metastatic cancer cell line –derived EVs induce apoptosis in senescent cells. In this meeting, we would like to discuss the biological significance of exosomes and exosomal miRNAs derived from senescent cells. miRNAs derived from Senescent cells.