Abstract

The Isolation and Analysis of Rare Cells, Exosomes, and Circulating Nucleic Acids For Liquid Biopsy

Daniel Chiu, Professor, University of Washington

This presentation will describe a rare-cell isolation instrument we call eDAR (ensemble decision aliquot ranking) for the automated enrichment and analysis of rare cells, a nanofluidic technology for the high-sensitivity and high-purity isolation of exosomes, and a digital-nucleic-acid detection platform based on our SD (self digitization) chip. I will outline the workings of these techniques, describe their performance, and discuss the clinical questions we are addressing to demonstrate the utility of liquid biopsy in precision medicine.