Abstract

Systematic Analysis of Human Pluripotent Cells

Jonathan Yuin-Han Loh, Principal Investigator, Institute of Molecular and Cell Biology A*STAR

The epigenome of differentiated cells is remarkably plastic. Cellular reprogramming or lineage conversions can be effected simply through the introduction of defined transcription factors. The ability to generate autologous, patient-specific stem cells offers unprecedented potential for disease research, drug screening, and regenerative medicine. We have performed comprehensive systematic analysis of the transcriptome, proteome, and phosphoproteome, evaluating multiple hESCs, fully reprogrammed hiPSC, and somatic lines. Our study reveals novel feature of RNA splicing mechanisms in regulating and maintaining the pluripotent cell state.