Abstract

High-Throughput Lung Microphysiological Systems

Shuichi Takayama, Professor, Georgia Research Alliance Eminent Scholar, and Price Gilbert, Jr. Chair in Regenerative Engineering and Medicine, Georgia Institute of Technology & Emory University School of Medicine

There is a need to better understand lung disease, accelerate drug discovery, and better predict human clinical trial outcomes and individualized drug responses. This presentation will describe efforts to develop microphysiological systems (MPSs) to accomplish this goal. Specific topics include the first microfluidic lung-on-a-chip developed at Michigan. A Transwell-96 based air-blood barriers that can be coupled with automated flow cytometry. And a 384 well format assay using lung Organoids with Reversed Biopolarity (lung-ORBs). Specific applications include single-ORB based high-throughput assays of SARS-CoV-2 infection and therapeutics, neutrophilic inflammation studies, and analyzing the injurious effects of fluid mechanical effects associated with lung stethoscope sounds.