Abstract

Translational Detection of Diverse Biomarkers Using Solid-State Nanopore Detectors

Adam Hall, Assistant Professor, Wake Forest School of Medicine

Nanopore technology is uniquely capable to probing large populations of molecules electrically and on an individual basis. While biological (i.e. protein-based) nanopores have found utility in commercial analytical technologies like DNA and RNA sequencing devices (e.g. Oxford Nanopore), their artificial counterparts have so far been less successful in finding a translational foothold. This is at least partially because few straightforward measurements have been demonstrated for the platform to analyze translationally or clinically important markers that cannot easily be probed with existing technologies. In this presentation, I will discuss our progress in developing solid-state nanopore assays capable of assessing several molecular targets that are difficult to probe by other means. These will include DNA epigenetic modification/lesions, microRNAs, and the glycan hyaluronan.