Rapid Detection of Pathogenic Bacteria on a Microfluidic Disc
Izumi Kubo, Professor, Soka University
We have developed an originally designed microfluidic disc-like device
with microchannels and microchambers for single cell isolation. For the
detection of genes in isolated cells on the device, hot cell-direct
polymerase chain reaction (PCR) method was invented. In this study, food
pathogenic bacteria such as Salmonella enterica was isolated on the
disc and determined by the amplification of its specific gene based on
hot cell-direct PCR using fluorescent probe. Relative fluorescence of
each microchamber after/before PCR was determined to detect the
bacteria. By the method, rapid and simple detection of bacterial cells
was performed.
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