Abstract

Screening a Pair of Aptamers using GO-SELEX for Nanobiosensors and Lateral Flow Assays

Man Bock Gu, Professor & Chair, Department of Biotechnology, Korea University

Aptamers are single-stranded nucleic acids having molecular recognition properties similar to antibodies, and isolated by in vitro selection and amplification process, SELEX. This talk will start with how the aptamers are innovatively screened, for the first time in the world, by using a new nano-material, graphene, without the immobilization of targets and how a few aptamer duos, inevitable for being applied in a sandwich-type platform, a reliable platform for commercialization eventually, were successfully develpoed for different pandemic influenza viruses, from graphene oxide-based immobilization-free screening (GO-SELEX).  In addition, some examples obtained from the interactions among aptamers, nanoparticles, and targets will be shown for a single or flexible aptamers with their successful implementation to the aptasensors. In addition, the benefits of using nano-sized materials for biosensing applications including lateral flow and SPR assays will be presented with scientifically proven clear examples of different organic-inorganic hybrid forms of nanomaterials and aptamers.