Abstract

Isolation, Discrimination and Transportation of Individual Specific Chromosomes From Single Mammalian Cells in a Microfluidic Channel

Hidehiro Oana, Associate Professor, The University Of Tokyo

Chromosomes were isolated from single mammalian cells in a microfluidic channel. Then, in the microfluidic channel, X chromosomes were fluorescently labeled by utilizing fluorescence-tagged dCas9 probes and discriminated. After that, capture/transportation of the X chromosomes were performed using optical tweezers.