Abstract

Microfluidics Within a 96-Well

Noo Li Jeon, Professor, Seoul National University

Conventional microfluidic devices based on Soft Lithography use channels formed by sealing a replicated PDMS structure against a transparent substrate such as a glass coverslip.  Although these devices have been widely used in laboratory setting, it has been challenging to adapt them for high-throughput drug screening applications due to material properties (small molecule absorption onto PDMS surfaces) and miniaturization problems.

This presentation will describe a new type of microfluidic device that is shaped like a 96-well and fit completely inside the well.  This device can be used to pattern 3D gels and cells for organ-on a chip applications.  We have successfully used this device to co-culture neurons and schwann cells as well as endothelial cells and fibroblasts and obtained results (myelination after long-term coculture and angiogenesis/vasculogenesis, respectively) similar to ones obtained in conventional microfluidic devices using PDMS.