Shopping Cart (0)
My Account

Shopping Cart
SELECTBIO Conferences Point-of-Care, Biosensors & Mobile Diagnostics Europe 2021


Combined Calorimetric Gas- and Spore-based Biosensor for Aseptic Food Processing

Michael Schöning, Director of Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences

Gaseous H2O2 represents one of the most used sterilization agents in aseptic food processing. To validate this sterilization method under investigation, robust spores that are resistant against this sterilant are first exposed. Afterwards, the logarithmic kill rate (i.e., how many survived from them) is determined by time-consuming (48-72 h) and tedious microbiological count-plating methods (e.g., count reduction test, end point test). A novel sensing method is introduced, consisting of a spore-based biosensor, which is combined with a calorimetric H2O2 sensor onto a single chip to evaluate both the viability of the spores and to determine the gaseous H2O2 concentration. The calorimetric gas sensor responds to different concentrations of gaseous H2O2 via change of its initial resistance, whereas the spore-based biosensor monitors the spore degradation, depending on the H2O2 concentration detecting the impedance variation. Three different strains of spores were investigated with regard to the H2O2 durability, namely B. atrophaeus DSM 675, B. subtilis DSM 402 and G. stearothermophilus DSM 5934. The two sensor types were combined as sensor array enabling a considerably more specific multi-parameter experience in aseptic filling machines in comparison to isolated microbiological state-of-the-art- or hydrogen peroxide methods.

Add to Calendar ▼2021-06-28 00:00:002021-06-30 00:00:00Europe/LondonPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2021Point-of-Care, Biosensors and Mobile Diagnostics Europe 2021 in Rotterdam, The NetherlandsRotterdam, The