Abstract

Introduction and Choosing and Validating Assay Systems to Interrogate 3D Culture Models

Terry Riss, Global Strategic Marketing Manager Cell Health, Promega Corporation

There is a broad spectrum of 3D cell culture systems being used to improve the physiological relevance and predictivity of in vitro models. I will describe key factors to consider when choosing a fit-for-purpose assay and mention the most commonly used methods applied to 3D culture models. Validating assay methods by using appropriate controls and multiplexing orthogonal assay methods will be presented.