Abstract

Biomaterials for Three-Dimensional Cellular Applications

Joshua Zimmermann, Senior Scientist, Stem Pharm, Inc.

Advances in three-dimensional culture systems have enabled the development of models that better capture the 3D nature of native tissue. Animal-derived extracellular-matrix products are commonly used for encapsulation or as scaffolds for these 3D models; however, these materials are generally undefined, variable, and have safety concerns due to their animal origin. Defined synthetic materials allow for more precise design and optimization of substrates for specific cellular applications that are free from animal derived components. Through control of properties such as substrate mechanical stiffness and adhesion ligand presentation, materials can be designed that enable more physiologically relevant extracellular environments and better direct cellular behaviors within 3D models. At Stem Pharm, we are applying these principles to design PEG-based hydrogels for the development of in vitro neural models.