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SELECTBIO Conferences Circulating Biomarkers, Exosomes & Liquid Biopsy Asia 2019


Microfluidic Chips Using Hydraulic Electric Analogy and Movable Layers for cfDNA Extraction and Amplification

Sung-Jin Kim, Associate Professor, Department of Mechanical Engineering, Konkuk University

We present two microfluidic chips that drastically reduce the use of dynamic external controllers for cfDNA extraction and amplification. The first chip generates pulsatile pressures to significantly reduce filter clogging without hemolysis, and consists of an oscillator, a plasma-extraction pump, and filter units. The oscillator autonomously converts constant gravity pressure to pulsatile pressure, and the pump uses the pulsatile pressure to extract plasma through the filter. In this way, we achieve plasma extraction with 100% purity and 90% plasma recovery at 15% hematocrit and without any significant filter clogging by red blood cells. This enables us to implement successful on-chip amplification of a nucleic acid (miDNA21) in plasma filtered from blood. The second chip has open chambers in vertically movable top layers and rotationally movable bottom layers to exploit surface tension in biochemical solutions, and implements control over fluid motion. This chip structure enables us to successfully manipulate solutions and magnetic beads without using on-chip valves and pumps that increase operational and structural complexity. The chip performs automated DNA extraction for cfDNA analysis.

Add to Calendar ▼2019-09-09 00:00:002019-09-10 00:00:00Europe/LondonCirculating Biomarkers, Exosomes and Liquid Biopsy Asia 2019Circulating Biomarkers, Exosomes and Liquid Biopsy Asia 2019 in Seoul, KoreaSeoul,