Abstract

A simple, sensitive in solution proximity ligation assay workflow for quantifying proteins in complex samples

Mark Shannon, Senior Staff Scientist, Thermo Fisher Scientific

Quantitative PCR (qPCR) has revolutionized the study of cellular nucleic acids, including genomic DNA, mRNA and non-coding RNAs, and has been extended to the analysis of proteins through the proximity ligation assay (PLA®). With its simple workflow and exquisite sensitivity, PLA® enables a broad range of applications including the quantification of proteins from cells, tissues, and blood samples along with the detection of post-translational modifications and protein complexes. I will discuss recent workflow enhancements, new applications, and key aspects of assay design, optimization, and data analysis.