Abstract

Multiplex Detection of Extremely Rare Mutant Sequences Associated with Cancer

Fred Kramer, Professor of Microbiology, Biochemistry & Molecular Genetics, New Jersey Medical School Rutgers University

Extremely specific “SuperSelective” PCR primers enable the simultaneous quantitation of extremely rare mutations that occur in different cells, even though they are located within the same or adjacent codons. Consequently, multiplex assays for the early detection of mutations associated with cancer should enable each patient’s therapy to be guided by the results of periodic “liquid biopsies” that analyze DNA fragments present in blood plasma samples.