Recombinant Human Collagen Based BioInk for 3D Bioprinting
Nadav Orr, Vice President, R&D, CollPlant
Collagen, the major component of connective tissues, is frequently
preferred for use in 3D printing of tissues and organs. Tissue extracted
collagen however suffers from batch-to-batch inconsistency, structural
damage, allergic response, risk of disease transmission, and exposure to
residual components from the source tissue. CollPlant’s technology of
engineered tobacco plants enables to produce a naïve human Type I
collagen (rhCollagen) with excellent rheological properties[1]. The
rhCollagen is thermally stable, preserves integrins and other essential
binding sites and demonstrates bioactivity resembling that of native
collagen. rhCollagen has excellent safety profile and shown superiority
in-vitro and in-vivo over tissue extracted collagens[2,3]. Medical
devices made of the rhCollagen were used to treat hundreds of humans in
the fields of advanced wound care and orthobiologics.
rhCollagen-methacrylate (rhCollagen-MA), curable by UV and visible
light, was developed for 3D Bioprinting, allowing printing at wide range
of conditions, including at room temperature. The unique viscosity and
shear thinning properties of rhCollagen-MA allowed the flexibility to
formulate BioInks compatible with all major printing technologies
including extrusion, ink-jet, laser and stereolithography. Scaffolds
made of rhCollagen-MA exhibited physical properties matching natural
tissues and demonstrated excellent support for cells proliferation and
vitality including a series of primary and differentiated human cells.
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