Abstract

EV Biomarkers from Resorbing Osteoclasts: A Complex Solution?

Shannon Holliday, Associate Professor, University of Florida

Recent studies have identified elements of EVs released by osteoclasts (microRNA-214-3p and receptor activator of nuclear factor kappa B) as circulating biomarkers for osteoporosis and psoriatic arthritis.  To identify additional candidates, we collected EVs from osteoclasts that were inactive (cultured on plastic) or resorbing (cultured on bone slices) and performed high-resolution 2D LC/MS analysis.  These data suggest that a number of different types of protein complexes are highly enriched in the EVs from osteoclasts.  Among these, a complex containing subunits of the vacuolar H+-ATPase (V-ATPase) is a strong candidate to serve as a biomarker for bone resorption.  We are currently validating V-ATPase subunit complexes and other protein complexes found in EVs as biomarkers for bone resorption in the gingival crevicular fluid.