Abstract

High-Throughput Characterization of Single Exosomes Using an Automated Platform

George Daaboul, Chief Scientific Officer, nanoView Diagnostics, Inc.

Current analytical tools have detection limitations when used for exosome characterization due to the small-size of exosomes. Specifically, exosomes have fewer available surface epitopes and low-signal relative to background levels, especially in complex samples. NanoView Biosciences has developed a label-free visible-light microarray imaging technique that allows multiplexed enumeration and sizing of individual exosomes and microvesicles captured on the sensor in a one-step assay direct-from-sample and can work with samples volumes as small as 5 µl. The technique is high-throughput and automated and can be used for standardization of exosome preparations, biomarker discovery, and for exosome-based liquid biopsies.