Subgroups of Extracellular Vesicles from Tumor Tissues for Biomarker Discovery and Liquid Biopsy Development in Malignant Disease
Jan Lötvall, Professor Krefting Research Centre, University of Gothenburg, Chief Scientist, Codiak BioSciences
Extracellular vesicles (EVs) have the capacity to shuttle both proteins,
lipids and nucleotides such as RNA between cells, leading to an array
of functional changes in a recipient cell. Importantly, the EV secretome
changes significantly in disease, especially in cancer. We have
recently developed a process to isolate EVs specifically from tumor
tissues, and have utilized this technology to identify an array of
biomarker candidates in malignant melanoma, breast cancer and colon
cancer. Specifically, the technique identifies EV surface molecules from
tumor tissue EVs, which are not present on plasma EVs from healthy
individuals. Using this information, we have been able to develop assays
to specifically identify cancer-EVs in the circulation in cancer
patients compared to healthy individuals. This presentation will discuss
EV diversity, and will give several examples of circulating cancer EV
biomarkers that can function as liquid biopsies in cancer subgroup
identification, cancer monitoring and putatively cancer screening.
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