Abstract

Plasma Extracellular Vesicles for Cardiovascular Disease

Dominique PV de Kleijn, Professor Experimental Vascular Surgery; Professor Netherlands Heart Institute, University Medical Center Utrecht

Cardiovascular Disease (CVD) is with the cardiovascular events of Ischemic Heart Disease and Stroke, the number 1 and 2 cause of death in the world and expect to increase especially in Asia. We are focussing on prognosis of secondary cardiovascular events after stroke and diagnosis of Ischemic Heart Disease. Ischemic heart disease (IHD) comprises 2 entities: Chronic Coronary Syndrome (CCS) and Acute Coronary Syndrome (ACS). Because CCS is associated with 6-8 times increased risk of adverse cadviovascular events like myocardial infarction and death, early recognition of CCS. Blood markers for CCS do not exist, resulting in that 80-90% of chest pain patients undergoing costly imaging do not have CCS. We use plasma extracellular vesicle protein content of vesicles from plasma subfractions as an accurate source for early diagnosis of CCS better then a clinical risk model. The same EV subfraction technology is used on plasma of stroke patients that after carotid atherectomy are at high risk of a second event (myocardial infarction, stroke or cardiovascular death). Protein as well as lipid content can identify patients at high risk for a secondary event better then a clinical risk prediction model.