Abstract

Multilevel Analysis of Individual EVs, Direct From Sample With Minimal Purification

Andrew Malloy, VP of Sales and Marketing, NanoView Biosciences

Current analytical tools have detection limitations when used for exosome characterization due to their small size.  Specifically, exosomes have fewer surface epitopes available and low-signal relative to background levels, especially in complex samples. Current workflows require multiple measurement technologies that result in the inability to link phenotypic and physical characterization on individual EVs across a biologically relevant size range. In addition, sample purification often results in loss of sample and technique dependent biases.  

NanoView Biosciences has developed an imaging technique that allows individual EVs as small as 40nm to be characterized direct from sample with no purification in most cases. Physical characteristics such as size and EV count can be presented alongside phenotypic information at the single EV level. Low volumes of sample can be probed and a panel of up to 10 surface markers can be interrogated in parallel, in a single measurement. Lastly, through the addition of fluorescent labeling, three additional markers can be probed and colocalized across the existing panel of 10 markers.  These capabilities make the tool ideally for basic EV research, biomarker discovery and the development of EV based liquid biopsies and therapeutics.