Abstract

Novel Origami Foil Based Microfluidic Foils for DNA Amplification and Multiplex Detection Fabricated by R2R UV NIL

Anne Linhardt, Scientist, JOANNEUM RESEARCH Forschungsgesellschaft mbH

Our pilot line allows patterning of microfluidic channels by R2R UV Nanoimprint Lithography (UV NIL), printing of biomolecules (nucleic acids; aptamers; proteins), by R2R microarray-spotting, and chip closing by R2R lamination. This manufacturing approach with a high degree of process parallelisation (e.g. 50 chips per imprinting tool, throughput of 3600 chip components per hour) leads to a faster and cheaper production compared to the injection moulded chips used so far by the diagnostic platform GENSPEED®. Regarding lab on foil, it is always a challenge to find enough space for liquids (sample, reagents, wash solutions) on the chip, especially if not only multiplex detection but also DNA amplification should fit on the chip size of 7.5 x 2.5 cm2. Therefore a novel origami technique for foil based diagnostic microfluidic chips was developed. After R2R UV NIL and R2R bio-functionalization, the foils with partially cured UV resin were folded and the layers connected by UV light irritation, leading to a chip with three microfluidic channel levels. This three-layer-chip was used to demonstrate on-chip amplification and subsequent detection of multiple analytes.