Abstract

Nuclear Magnetic Resonance Based Metabolomics for the Biomarker Discovery in Gallbladder Cancer and Acute Respiratory Distress Syndrome (ARDS)

Neeraj Sinha, Professor, Sanjay Gandhi Postgraduate Institute of Medical Sciences

Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) based spectroscopic techniques has become an indispensable tool for the metabolomics study in recent years. While sensitivity of MS based methods are more than NMR spectroscopy, there are many advantages with NMR based metabolomics study. NMR provide advantages such as high reproducibility, least sample preparation procedure and accuracy in quantitative estimates of the metabolites. Typical bio fluids such as serum, urine, whole blood cell, bronchoalveolar lavage fluid (BALF) etc can be subjected to NMR spectroscopy for accurate detection of metabolites. The multiparametric response to the system can be mapped in terms of NMR spectral density. The interpretation of these responses can give insight into the metabolic cycle of disease condition.

This talk will highlight some of these methods developed in our laboratory for gallbladder cancer, acute respiratory distress syndrome (ARDS) and acute lung injury (ALI).