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SELECTBIO Conferences Microfluidics for Circulating Biomarkers Summit 2019


Negative Enrichment and Gene Expression Analysis of CTCs by Using a Microfluidic Device

Liang Zhao, Assistant Professor, University of Science and Technology Beijing

Traditional methods for CTCs capture and analysis are usually utilize speci?c antibody to isolate CTCs from periphery blood. However, some circulating tumor cells that are undergoing epithelia-mesenchymal transformation may lose its surface antigen which was speci?cally expressed in epithelial cells. Herein, we developed a micro?uidic device for capturing CTCs and analysis without bias from whole blood by negative depletion of red blood cells (RBCs) and white blood cells (WBCs) off-chip. The operation is ultimately easy and interested CTCs could be distinguished and retrieved for further molecular analysis.

Add to Calendar ▼2019-10-10 00:00:002019-10-10 00:00:00Europe/LondonMicrofluidics for Circulating Biomarkers Summit 2019Microfluidics for Circulating Biomarkers Summit 2019 in Coronado Island, CaliforniaCoronado Island,