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SELECTBIO Conferences Innovations in Microfluidics 2020

Abstract



A Systematic Comparison of Single Cell RNA-Seq Methods

Joshua Levin, Senior Scientist/Group Leader, The Broad Institute of MIT and Harvard

A multitude of single-cell RNA sequencing methods have been developed in recent years, with dramatic advances in scale and power, and enabling major discoveries and large scale cell mapping efforts. We directly compared seven methods for single cell and/or single nucleus profiling from three types of samples – cell lines, peripheral blood mononuclear cells and brain tissue. To analyze these datasets, we developed and applied scumi, a flexible computational pipeline that can be used for any scRNA-seq method. We evaluated the methods for both basic performance and for their ability to recover known biological information in the samples.


Add to Calendar ▼2020-08-17 00:00:002020-08-18 00:00:00Europe/LondonInnovations in Microfluidics 2020Innovations in Microfluidics 2020 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com