Abstract

Chromosome conformation capture techniques (often abbreviated to 3C technologies or Hi-C or 3C) are a set of molecular biology methods used to analyze the spatial organization of chromatin in a cell. With high throughput sequencing it becomes possible to capture the spatial organization of the genome by analyzing at the alignment of discordant read pairs. We develop a method to obtain the 3-D organization of a genome using this method and apply it to the C. crescentus genome.