Abstract

Exploring in vitro Vibrational Microspectroscopy for High Content Analysis

Hugh Byrne, Professor, Dublin Institute of Technology

The potential of vibrational spectroscopy, but infrared absorption and Raman scattering, in the fields of biological and biomedical applications has been well demonstrated. The techniques provide molecular based profiles of the biomolecular content, in a label free, non-destructive manner, which, in the microscopic mode, can have subcellular resolution. Multivariate statistical analysis is commonly employed to extract high content information concerning cellular metabolism and response pathways. The presentation provides an over view of efforts to advance the application of vibrational spectroscopy for high content cellular analysis, using examples of exposure to model nanoparticles and chemotherapeutic agents. This progress is discussed in the light of emerging technologies in the field of spectroscopic microscopy.