Abstract

Integrated Optofluidic Platform for Single Bioparticle Analysis

Holger Schmidt, Narinder Kapany Professor of Electrical Engineering, University of California-Santa Cruz

We have developed an optofluidic platform that integrates biological sample preparation and optical single molecule analysis on a chip. I will describe the principle of the platform based on liquid-core optical waveguides and a broad range of capabilities that are enabled by this approach. These include the fluorescence analysis of single nucleic acids for amplification-free detection of Ebola infection as a new paradigm for molecular diagnostics with ultrahigh sensitivity and dynamic range. I will also describe multiplexed target detection with multi-mode interference waveguides for identification of single influenza viruses. The expansion of optofluidic capabilities through hybrid integration via nanopore-based electrical detection and integrated sample processing will be discussed.