Registration

Next Generation Sequencing on Microarrays
Kalim Mir, Visiting Scholar, University of Oxford, United Kingdom

I will show how microarrays can be used as a platform for next generation sequencing and how single molecules can be detected within microarray features.

Automatic Algorithms for Analysis of cDNA Microarray and Chip-seq Data
Luis Rueda, Associate Professor, University of Windsor, Canada

This presentation discusses the new trends on automatically processing cDNA microarray images using clustering algorithms. Applications to processing Chip-Seq data to automatically detect binding sites are also discussed.

Coffee Break and Networking in Exhibition Hall

In Situ. Light- Directed Synthesis of High-Density RNA Microarrays
Mark Somoza, Professor, University of Vienna, Austria

High-density RNA microarrays, with hundreds of thousands of sequences, would be very useful for studying molecules that specifically interact with RNA—such as RNA-binding proteins, ribonucleases and drugs targeting RNA—and to make aptamer arrays for diagnostic applications.

Technology Spotlight:
Ingenuity iReport for Gene Expression Analysis
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Megan Laurance, Associate Staff Scientist, Ingenuity Systems

Lunch Break and Networking in Exhibition Hall

Free Workshop
Single Sample, Single Slice, Single Prep, Single Well… Multiple Answers

Poster Viewing Session

Gene Expression Profiling of Human Myeloid Leukemic MV4-11 cells Treated with 5-aza-2’-Deoxycytidine
Kyu-Tae Kim, Research Fellow, University of Newcastle, Australia, Australia

In this study, we have treated a human myeloid derived leukemic MV4-11 cell with a demethylating agent 5-aza-2'-deoxycytidine to examine its influence on genome wide gene expression. Whole genome expression profiles were measured using llumina Human Ref-8 arrays.

Using Microarrays to Understand the Developmentally Complex Regulation of the Intestinal Fructose Transporter GLUT5
Rolando Ferraris, Professor, UMDNJ, United States of America

Fructose induces rat GLUT5 expression only during weaning. We segregated age- and fructose-responsive genes and showed that GLUT5 regulation involves cross-talk between nutrient signals regulating specific transporters and hormonal, particularly glucocorticoid receptor-mediated, signals modulating intestinal maturation.

Coffee Break and Networking in Exhibition Hall

New Technologies for Large Scale Manufacturing of High Density Arrays. Design Rules and Cost Drivers
Georg Bauer, Head of R&D, Sony DADC, Germany

High density well and pillar arrays are applied from next generation sequencing to bead-based screening assays and clinical chemistry. The talk will address technical limitations, describes basic design rules and cost drivers.

Automating the Analysis of Tissue Microarrays in Basic Science, Clinical and Translational Applications
Toby Cornish, Assistant Professor, Johns Hopkins Medicine Bayview Medical Center, United States of America

Fully-automated segmentation is one of the few remaining obstacles to realizing the tissue microarray (TMA) as a truly high-throughput technique for tissue analysis. Approaches to automatic segmentation will be discussed, including multiplexing of reporters and pattern recognition.

Drinks Reception

Actionable Biomarkers for Rheumatoid Arthritis
William Robinson, Associate Professor, Stanford Univeristy School of Medicine, United States of America

We have developed multiplex proteomic tests to guide therapeutic decision making in rheumatoid arthritis (RA). Through proteomic discovery, we have identified autoantibody and cytokine profiles with predictive utility for two actionable situations in RA. First, to facilitate early diagnosis and initiation of disease-modifying therapies that alter the natural history of RA. Second, to identify patients likely to respond to anti-TNF therapeutics.

Via Pre-Recorded Presentation A Systematic Investigation of Candidate Breast Cancer Progression-Associated Biomarkers Identified from Omic Screens: Leveraging Antibody-Based Proteomics
William Gallaghr, Chief Scientific Officer/Professor, OncoMark Limited/University College Dublin, Ireland

A key bottleneck in the translation of discoveries from transcriptomic and proteomic screens into clinically viable diagnostics relates to current deficits in relation to antibody availability. This presentation provides a case example of how antibody-based proteomics can be applied to transition candidate breast cancer progression-related biomarkers from such omic screens.

Coffee Break and Networking in Exhibition Hall

Tumor Autoantibodies as Biomarkers for Predicting Ovarian Cancer Recurrence
Madhumita Chatterjee, Research Scientist, Wayne State University School of Medicine, United States of America

We performed a study on sera from ovarian cancer patients, benign and healthy individuals to identify biomarkers that can predict ovarian cancer diagnosis and recurrence. We will present a validation study using our novel antigens and 13 known tumor antigens.

Protein Microarray Technologies - Analysis of Signaling Networks in Tumours
Markus Templin, Head, University of Tuebingen, Germany

Complex data sets generated from limiting sample material allow the identification of differences in activation of signaling networks directly on the protein level. Protein microarray analysis during anti-cancer drug development will demonstrate the power of the technology.

Technology Spotlight:
The Automation of Microarray Processing
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Peter Herzer, Senior Applications Scientist, Tecan

Lunch Break and Networking in Exhibition Hall

Poster Viewing Session

Protein and Peptide Arrays for Studying Autoimmunity
Paul Utz, Associate Professor, Stanford University School of Medicine, United States of America

Protein and peptide microarrays have tremendous potential for many applications in biology and in medicine. The speaker will present data demonstrating the utility of protein and peptide arrays for characterizing autoantibodies, identifying enzyme substrates, and understanding the epigenetic code.

Antibody Arrays as Diagnostic Tool for Agricultural Research and Development
Nitsara Karoonuthaisiri, Head of Microarray Laboratory, National Center for Genetic Engineering and Biotechnology, Thailand

Antibody arrays were developed for a variety of agricultural applications. Two examples of antibody arrays for detection of foodborne and seedborne pathogens will be presented. These antibody arrays based on mini sandwich ELISA were fabricated in a 96-well plate format.

Coffee Break and Networking in Exhibition Hall

Protein Kinase and Phosphosite Biomarker Discovery and Validation with Protein Microarrays with Antibodies, Lysates, Protein Kinases, and Substrate Peptides
Steven Pelech, President/Chief Scientific Officer/Professor, Kinexus Bioinformatics Corporation, Canada

Kinexus identified 650,000 known/predicted human phospho-sites, investigated their evolutionary conservation, and deduced the substrate specificities for 500 kinases. A unique suite of microarrays with antibody, cell/tissue lysates, protein kinases, and peptides were created to experimentally test these bioinformatics predictions.

Close of Conference