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From Single Cell Signaling Analysis to Simulated Physiological Systems: Advances in the Application of Microfluidic Technology Towards Deciphering Today’s Biological Mysteries
Shannon Faley, Research Fellow, Vanderbilt University, United States of America

This talk, based upon the past 8 years of implementing our microfluidic nanophysiometer towards understanding adaptive immune response, response of leukemic cells to chemotherapeutic agents, and stem cell mediated cardiac repair following myocardial infarction, aims to demonstrate such microfluidic platforms as a powerful means for understanding important processes on a cellular level. The future of microfluidic technology, including integration with additional detection modalities to creating better system models, will also be discussed.

Single Cell and Subcellular Metabolic Analysis by Mass Spectrometry
Akos Vertes, Professor, George Washington University, United States of America

In this presentation two new ionization platforms are described for the direct analysis of single cells and subcellular components: an ambient method called laser ablation electrospray ionization (LAESI) and an ultrasensitive nanophotonic ion source based on silicon nanopost arrays (NAPA).

Coffee Break and Networking in the Exhibition Hall

Uncovering New Stem Cell Sub-Populations: A Novel High-Throughput Single Cell Analysis Technology
Ivan Dimov, Instructor, Stanford University, United States of America

Using a 20 pico-liter-well array we have demonstrated the first discrimination of sub-populations based on gene-expression measurements. Measuring over 300 single-cell observations is essential to overcoming experimental noise. Analyzing pure HSCs, we have identified genes candidates that fractionate the population.

Neurometabolomics: The Cell by Cell Chemical Characterizations of the Brain
Jonathan Sweedler, Director, University of Illinois, United States of America

The chemical complexity of many cell samples is surprisingly high so that measures of the average values of the constituents from populations of cells obscures critical information about select cells. Several single cell measurement approaches are highlighted to measure the cellular metabolome.

A Workflow for Single-Cell Resolution, Automated, Image-Based Sorting of Pure Circulating Tumor Cells and Their Comprehensive Molecular Characterisation
Nicolò Manaresi, Chief Technology Officer, Silicon Biosystems, Italy

Some of the key challenges to unlock the potential of Circulating Tumor Cells (CTC) in personalized therapy and translational research are related to the low level of purity provided by enrichment techniques, and the small number of CTCs available. We demonstrate a methodology addressing these challenges, based on the DEPArray™ system to sort multiple individual 100%-pure CTCs, followed by Whole Genome/Transcriptome Amplification for their molecular analysis.

Lunch and Networking in the Exhibition Hall

Free Workshop
Single Sample, Single Slice, Single Prep, Single Well… Multiple Answers

Poster Session

Single Cell Microarray of Mouse Cardiomyocytes During Aging and in a Novel Model of Cardiac Oxidative Stress
James Flynn, Field Application Scientist, NextBio, United States of America

This presentation will entail single cell microarray and quantitative PCR to defining a novel model of heart disease. heart function is used as a metric by which we are correlating expression changes and stochastic variance between single cells.

Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with DNA barcoding
Rong Lu, Postdoctoral Scholar, Stanford University, United States of America

We present a novel experimental system combining viral cellular barcoding with high-throughput sequencing to track single cells in vivo development. It can be applied to the clonal tracking of in vitro or in vivo processes for virtually any cell type.

Coffee Break and Networking in the Exhibition Hall

RT-qPCR in the Analysis of Single Circulating Tumor Cells
Katharina Pachmann, Professor, University of Jena, Germany

Determination of the eterogeneity of circulating tumor cells at the single cell level will allow pharmaceutical companies ro characterize thse cells as true targets of systemic therapy and design the respective drugs according to targets expressed on these cells.

Digital CastPCR for Direct Detection of Circulating Tumor Cells in Cancer Patients
David Deng, Senior Manager, Life Technologies, United States of America

Use digital cell-by-cell analysis for direct detection of circulating tumor cells in cancer patients using castPCR has a great potential in early cancer detection and care.

Toward Multiplexed Sequencing of Single CTCs and DTCs Using Next-Gen Sequencing
John Langmore, Chief Scientific Officer, Rubicon Genomics Inc, United States of America

Next-generation sequencing of single cells must be accurate, reproducible, rapid, and inexpensive for use in research and diagnostics. Indexed PicoPlex NGS preps of cloned cancer and normal cells gave accurate, reproducible, and inexpensive multiplexed copy number and mutation analysis.

Technology Spotlight:
Identifying and Characterizing Subpopulations by Single Cell Analysis
Richard Fekete, Senior Manager, Life Technologies

Small subpopulations of cells are often lost when looking at large numbers of cells in each sample. These cells are often very important phenotypically, and can provide a great deal of information about the fate or impact of the entire population. Using single cell analysis these cells can be identified and thoroughly characterized.

Drinks Reception

Liquid Biopsy of Ovarian Cancer: Issuing Diagnosis and Planning Therapies of Ovarian Cancers Based Upon Genome Sequencing and Gene Expression Profiling of Single Cells From Peritoneal Fluid and Blood
Marek Malecki, President, Phoenix Biomolecular Engineering Foundation, United States of America

Cancers of the ovaries are the most malignant among gynecological neoplasms. The ultimate goal of this project is defining molecular biomarkers of single cancer cells, which would allow us for the earliest diagnosis followed by the targeted therapy.

Genome-Scale Analysis With Single Cells
Honghua Li, Associate Professor, UMDNJ Robert Wood Johnson Medical School, United States of America

A newly developed high-throughput experimental system has made it possible for both genetic and gene expression profiling of single cells on a genomic scale and has been successfully used for a number of applications.

Coffee Break and Networking in the Exhibition Hall

Single-Cell-Kinetics Approach to Study Multidrug Resistance
Sergey Krylov, Professor, York University, Canada

We introduced a single-cell approach to study kinetics of multidrug resistance (MDR) and found that cancer cells in the later phases of the cell cycle have greater activity and efficiency of transporters responsible for MDR-associate drug efflux.

Manipulating Bacterial Growth for Single Cell Genomics
Armand Dichosa, Postdoctoral Research Associate, Los Alamos National Laboratory, Mexico

We present a novel approach to improve genomic recovery from a single cell after whole genome amplification (WGA) via multiple displacement amplification (MDA). We also present ongoing research demonstrating LANL Bioscience’s capabilities to further single cell genomics.

Lunch and Networking in the Exhibition Hall

Poster Session

Investigate Cell Cycle Regulation with Single-cell Molecular Analysis
Jiang Zhong, Assistant Professor, University of Southern California, United States of America

I will talk about a new approach of investigating stepwise biological events without the need of biomarkers. With a microfluidic platform for single-cell molecular analysis and bioinformatics analysis, we develop a new method to construct molecular maps that describe the stepwise gene network perturbation.

Physical and Chemical Analyses of Single Cells on Microfluidic Platforms
Samuel Kim, Zare Lab, Stanford University, United States of America

I will present recent advances from our research group in the field of single-cell analysis using microfluidic devices, including genomic analysis of thermophilic cyanobacteria from environmental samples and sorting of mammalian cells based on multilayered porous membrane structures.

Coffee Break and Networking in the Exhibition Hall

Microfluidic Single Cell Analysis Platforms for Ensemble Measurements of Cell-Signaling Cascades
Bryan Carson, Senior Member of Technical Staff, Sandia National Laboratories, United States of America

This presentation will focus on detailing the microfluidic hardware developed to study immune response in single cells. Several platforms will be highlighted, including an optical interrogation platform and an impedimetric interrogation platform.

Single Cell Separation and PCR on Compact Disk (CD) Shaped Microfluidic Device
Izumi Kubo, Professor, Soka University, Japan

We have performed single cell isolation and PCR on our original CD-shaped microfluidic device. PCR of inv A gene in S. enterica cells and RT-PCR of the m-RNA in Jurkat Cells are demonstrated in this paper.

Close of Conference