Thursday, 12 September 2013


Single Cell Analysis (SCA) - Current Technology Trends

09:00

Richard MathiesKeynote Presentation

High Throughput Technologies for Single Cell Genetic Analysis
Richard Mathies, Professor, University of California Berkeley, United States of America

Using emulsion PCR and RT-PCR, we are able to determine the sequences and frequencies of genetic mutations and changes in gene expression at the single cell level. 

10:00

Coffee Break and Networking in the Exhibition Hall

10:45

Single Cell Analysis of Senescent Cells
James Flynn, Research Associate, Buck Institute for Research on Aging, United States of America

As we age, our cells undergo a process where they enter a senescent state as a potent tumor suppression mechanism, but this cellular change may also contribute to the aging pathology seen within tissues including inflammation and bioenergetic compromise. In this talk the use of single cell analysis is used to dissect the cellular transition from a quiescent to senescent state.  

11:30

In Vivo Single Cell mRNA Analysis
James Eberwine, Professor, University of Pennsylvania, United States of America

12:15

Lunch Break and Networking in Exhibition Hall

13:30

Poster Viewing Session


CTC's - A Clinically-Relevant SCA Paradigm

14:15

Single Cell Transcription Profiling to Better Understand Developmental Decisions
Steve Potter, Professor, Cincinnati Children's Hospital Medical Center, United States of America

Microfluidic/robotic approaches allow the high throughput RNA-Seq transcriptome analysis of single cells. We describe the use of the Fluidigm system to study single cell heterogeneity in developing organs.  
 

14:45

Alan NelsonKeynote Presentation

Breakthrough Cell-CT for Early Diagnosis of Lung Cancer from Sputum and Blood
Alan Nelson, Professor, Arizona State University , United States of America

15:45

Coffee Break and Networking in the Exhibition Hall

16:30

Daniel ChiuKeynote Presentation

Counting and Analysis of Individual Circulating Tumor Cells
Daniel Chiu, A. Bruce Montgomery Professor of Chemistry, University of Washington, United States of America

This presentation will describe a technology platform, termed eDAR, that we have developed in the past years for the isolation, enumeration, and analysis of individual rare cells and circulating tumor cells from peripheral blood.

17:30

Large-scale Microchamber Array Allows High-throughput and Parallelized Readouts of Cellular Heterogeneity
Soo Kim, Research Associate, University of Tokyo, Japan

We have developed a high-throughput method for quantitative analysis of intracellular materials of single cells by using arrayed microchambers. The feasibility of our approach was demonstrated by measuring total amount of intracellular ATP molecules in individual cells.

18:15

Reception and Roundtable Discussions in Exhibition Hall

19:30

End of Day One

Friday, 13 September 2013

08:30

NanoVelcro-Embedded Chips that Enable Molecular Analysis of Single Circulating Tumor Cells
Hsian-Rong Tseng, Professor, Crump Institute for Molecular Imaging, California NanoSystems Institute, University of California-Los Angeles, United States of America

This presentation will introduce a new category of NanoVelcro Chips capable of not only capturing circulating tumor cells (CTCs) with high efficiency, but also enabling highly specific isolation of single CTCs immobilized on the embedded nanosubstrate without contamination by white blood cells (WBCs).  The individually isolated CTCs can then be subjected to mutation analysis by next generation sequencing, providing insights to molecular mechanisms responsible for the malignant transformation in cancer.

09:15

Selection and Molecular Profiling of Circulating Tumor Cell (CTC) Sub-populations: Single-Cell Studies of Epithelial-to-Mesenchymal Transitions
Steven Soper, Professor, Louisiana State University, United States of America

Rare CTC sub-populations can be isolated directly from whole blood and processed for molecular signatures at the single-cell level to identify key drivers of epithelial-to-mesenchymal type transitions that may be associated with metastatic disease.

10:00

Coffee Break and Networking in the Exhibition Hall

10:45

CANCELLED - DUE TO INTERNAL COMPANY ISSUES
Rajan Kumar, President, Genome Data Systems, Inc., United States of America


Putting SCA to Work in the Clinic

11:30

Mass Spectrometry Imaging of Single Cells in Mouse Models of ALS
Jeffrey Agar, , Brandeis University, United States of America

Techniques for the MALDI mass spectrometry imaging of single mouse neurons, specifically those in mouse models of neurodegenerative disease, are presented.

12:15

Lunch Break and Networking in Exhibition Hall

13:30

Poster Viewing Session

14:15

Optical Sensors for Quantification of Intracellular Redox Potentials
Colin Campbell, Senior Lecturer, University of Edinburgh, United Kingdom

This presentation will focus on recent advances in the development and use of optical sensors for the study of intracellular redox potential. Some examples of the use and interpretation of these sensors will be given. 

15:00

Microengineered Devices for Single Cell Analysis in Preclinical and Clinical Research
Nancy Allbritton, Frank and Julie Jungers Dean of the College of Engineering and Professor of Bioengineering, University of Washington in Seattle, United States of America

Microanalytical platforms in both array and microfluidic formats are being developed to address critical preclinical and clinical needs to accurately monitor drug action and identify the patient who will respond to new therapies aimed at modulating signal transduction pathways.

15:45

Coffee Break and Networking in the Exhibition Hall

16:15

Clonal Analysis of Cancer at the Single Cell Level
James Hicks, Research Professor, Cold Spring Harbor Laboratory, United States of America

Nearly all solid tumors consist of heterogeneous mixtures of genetically abnormal and genomically unstable cells.  As such, the basic unit of the disease is the single cell.  The goal of our work is to enable the molecular dissection of cancer at the single cell level and to extract the maximum useful information in the most cost efficient manner.  We will discuss methods for rapid CNV analysis and focal resequencing for SNV as applied to circulating tumor cells and core biopsies.

17:00

Single Cell RNA Seq Analysis of Mammalian Preimplantation Development
Guoping Fan, Professor, University of California Los Angeles, United States of America

We will highlight the recent development in single cell RNA-seq analysis and provide a case study of the utility of this technique in the analysis of gene expression network in mammalian preimplantation development.

17:45

Market Analysis of Single Cell Analysis: Segmentation, Sizing, and Opportunities
Enal Razvi, Managing Director, Select Biosciences Inc, United States of America

SELECTBIO has been tracking the various market segments that constitute the single cell analysis space and have been collecting market data relating to several active segments within this field.  In this presentation, I will provide a snapshot of the market landscape, SWOT, and competitive analysis of this fast-moving field.

18:30

Close of Conference