08:00 | Registration |
| Day One Session |
| Session Chair: Alexander Kapustin, Research Associate, King's College London, United Kingdom |
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09:00 | Standardisation of Extracellular Vesicle Measurements Edwin van der Pol, Postdoctoral Researcher, Amsterdam University, Netherlands
Extracellular vesicles are heterogeneous in all their aspects, they are isolated using different protocols, and they are measured by different techniques. Therefore, data interpretation and comparison is challenging. This lecture focuses on standardization of extracellular vesicle isolation and detection. |
09:30 | |
10:15 | Large Oncosomes: New Frontiers for Cell-to-Cell Communication in Cancer Dolores Di Vizio, Professor, Cedars Sinai Medical Center, United States of America
Large oncosomes are a distinct type of extracellular vesicles that originate from the shedding of bulky membrane blebs. The abundance of large oncosomes in the circulation and in tissues correlates with advanced disease in mouse models and patients with cancer. Large oncosomes represent a novel population of EVs and valuable candidates for identification of new cancer-specific biomarkers. |
10:45 | Coffee & Networking |
11:45 | Therapeutic Potentials of Mesenchymal Stem Cell-derived Extracellular Vesicles Bernd Giebel, Group Leader, Institute for Transfusion Medicine, University Hospital Duisburg-Essen, Germany
MSCs have been used to treat a variety of different diseases such as myocardial infarction, stroke and graft-versus-host disease (GvHD). Initially, MSCs were thought to replace lost cells in damaged tissues. Despite contrary reports regarding the outcome of MSC treatments, MSCs seem to exert their beneficial effects by the secretion of immunosuppressive factors and/or small extracellular vesicles (EVs, 70-150 nm), such as exosomes and microvesicles, rather than by interacting into affected tissues. After setting up techniques for the characterization and large scale preparation of EVs, we have treated a steroid-refractory GvHD patient with EVs/exosomes enriched from MSC supernatants (MSC-EVs). In addition we investigate the MSC-EVs’ therapeutic potential to exert neuroprotective functions in animal models for ischemic stroke and preterm brain injury. So far, we observed beneficial effects in all settings. In the murine stroke model we performed a side by side comparison of the therapeutic effect of MSCs and their EVs and did not recognize any differences, both improved the symptoms significantly. At the functional level MSC-EVs were shown to exert immunosuppressive functions in vivo and in vitro. |
12:15 | Production of Functionally Bioactive EVs from an Immortalized Human Neural Stem Cell (hNSC) Line Randolph Corteling, Head of Research, ReNeuron Ltd., United Kingdom
To ensure the large scale required for clinical research and development, producer cell immortalization and clonal isolation is a practical strategy to produce consistent, functionally bioactive exosomes for use as therapeutic agents.
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12:45 | Lunch & Networking |
13:30 | Poster Viewing Session |
14:00 | | Keynote Presentation Oncogene Transmission by Extracellular Vesicles Janusz Rak, Professor, McGill University, Canada
Oncogenes may promote their own intercellular trafficking as cargo of extracellular vesicles (EVs) produced by cancer cells. These processes will be discussed, including the extent, impact and barriers for horizontal oncogenic transformation, as well as the related diagnostic implications. |
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14:45 | The Significant Function of EVs in Aging and Cancer Hidetoshi Tahara, Deputy Executive Director, Industry-Academia Collaboration; Professor, Department of Cellular and Molecular Biology; Head, The Research Center for Drug development and Biomarker Discovery, Hiroshima University, Japan
The secretion of EVs from senescent fibroblast cells may contribute to tumor microenvironment. Senescent cells and cancer cells may be fighting each other by shooting own EVs. |
15:15 | Coffee & Networking |
15:30 | Long Distance Signaling Between Tissues Via Extracellular Vesicles Stefan Momma, Group Leader, University Hospital Frankfurt, Germany
In this talk we will present a novel method to investigate the extent and function of EV mediated signalling in vivo. |
16:00 | Extracellular Vesicles from Basic Biology to Next Generation Therapy: A Translational Perspective Ahmed Ibrahim, Research Scientist, Capricor Therapeutics, United States of America
Extracellular vesicles (EVs) are nano-sized lipid bilayer particles secreted by all cells and transmit bioactive signals including proteins and RNA. EVs secreted by Cardiosphere-derived cells regenerate damaged tissue and currently being developed as a next generation therapeutic product. |
17:00 | Punting on the River Cam The tour takes in seven riverside colleges and nine famous bridges, the Punt Chauffeur will give an entertaining commentary and history overview of each. |
17:45 | End of Day One |