08:00 | Registration |
|
Session: MIQE and qPCR Accuracy |
| |
09:15 |  | Keynote Presentation MIQE Compliance in qPCR
Stephen Bustin, Professor, Anglia Ruskin University, United Kingdom
This presentation provides evidence that substantiate the perception that many publications relying on qPCR data need to be treated with caution, especially when published in high impact factor journals. |
|
09:45 | Measurement Accuracy in Single Cell Gene Expression Analysis
Alison Devonshire, Science Leader, LGC Limited, United Kingdom
Single cell RT-qPCR reveals cellular heterogeneity within populations, however defining measurement precision is challenging. We have assessed strategies for quantifying sources of technical variability and bias involved in cell capture, extraction, reverse transcription, pre-amplification and qPCR and demonstrate how RNA standards can be used for quality control. |
10:15 |  Technology Spotlight:
Automating Various Genomic Workflows On One Single Platform, the Agilent Bravo
Ingo Poleschak, Product Specialist, Agilent Technologies
This presentation will give you a short overview about how you could realize workflows for your DNA/RNA-extractions, PCR- and qPCR, CCH, NGS and target enrichment experiments, this for low, mid- or high throughput, using Agilent Technologies flexible automation toolkit. We provide turn-key solutions as well as an open platform which leaves you the choice of many different kits or frequent changing protocols. With its pipetting range from 0.3ul to 250ul and its single tip capability, the Agilent Bravo Liquid Handler is the instrument of choice to realize all your workflows in an easy to use, reliable and flexible manner. Let us take you on a fascinating journey into modern genomics lab automation.
|
10:30 | Coffee Break and Networking in the Exhibition Hall |
|
Session: Single Cell qPCR |
| |
11:15 | Understanding Cell Heterogeneity at Single-Cell Level
Anders Stahlberg, Senior Scientist, University of Gothenberg, Sweden
o Technical and biological aspects of single-cell gene expression profiling
o Strategies to analyze single-cell data and identify unknown subpopulations
o Single cell biology and interpretations |
11:45 | Single Cell Expression Profiling – Revealing Astrocyte Cell Subtypes by Correlation
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden
In my talk I will describe single cell expression correlation as means to classify cell subtypes. The technique exploits the correlation between expression levels of transcripts involved in related key biological processes within individual cells. The technique is used to reveal subtypes of astrocytes. |
12:15 |  Technology Spotlight:
Real-time PCR Innovations
Laura-Leena Kiiskinen, R&D Manager, Thermo Fisher Scientific
Thermo Scientific provides the modern researcher with a broad portfolio of innovative products for real-time PCR experiments. A few examples of recent reagent developments include protein engineering of enzymes utilized within reverse transcription and qPCR products to deliver performance improvements in gene-expression and SNP genotyping studies, as well as the introduction of a three colour preparation system to support error-free sample handling. Combined with industry-leading Thermo Scientific PCR and qPCR plastic consumables, these innovations deliver high quality real-time PCR experimental data with minimal protocol times.
|
12:30 | Lunch and Networking in the Exhibition Hall |
12:45 | Free Workshop
Improving Your qPCR Results: A Systematic Approach to Understanding qPCR Detection |
13:30 | Poster Viewing Session |
|
Session: qPCR in Diagnostics |
| |
14:15 | HPV Strain Type Diagnosis by Sequencing qPCR Amplicons Via Hybridisation to Tiling Arrays
Philip Day, Reader, Manchester University, United Kingdom
Co-infections and genetic polymorphisms with papilloma viruses compromise precise strain type analyses, and therefore HPV qPCR amplicons were subjected to hybridisation to a tiling oligonucleotide array to improve detection including multiple strain typing. |
14:45 | Nucleic-Acid Based Applications in the Diagnostics of Highly Pathogenic Viruses
Andreas Nitsche, Head of the ZBS 1, Robert Koch Institute, Germany
The presentation describes the various approaches that can be considered for the unambiguous identification of highly pathogenic agents by PCR based techniques. Beside design strategies, questions of identification and sample preparation are discussed. |
15:15 | Coffee Break and Networking in the Exhibition Hall |
|
Session: Biostatistics & Bioinformatics |
| |
16:00 | Quantitative PCR Data Analysis with Efficiency Values Derived from Amplification Curves.
Jan Ruijter, Research Group Leader, University of Amsterdam, Netherlands
The presentation will describe the significance of the correct estimation of baseline fluorescence, the estimation of efficiency values from individual amplification curves for different monitoring chemistries and the effect of primer concentration on the amplification efficiency. |
17:00 | Visualizing High-Throughput qPCR Data: Novel Heatmaps, Scatter Matrices and 3D Plots
Andrej-Nikolai Spiess, Group Leader, University Hospital Hamburg Eppendorf, Germany
In the age of high-throughput data, specialized data visualization techniques are required to understand structures and tendencies in the data. In respect to qPCR technology, we have developed some plot types that reveal success/failure of qPCR runs. |
17:30 | Drinks Reception |
