Tuesday, 5 March 2013

08:00

Registration


PCR and NGS Expression Profiling

09:00

Anders StahlbergKeynote Presentation

Quantitative PCR Analysis of DNA, RNAs, and Proteins in the Same Single Cell
Anders Stahlberg, Senior Scientist, University of Gothenberg, Sweden

In this presentation we will demonstrate how DNA, RNAs, and Proteins can be analyzed in the same single cell. Multi-parametric correlation studies at single cell level open up new possibilities to understand dynamic biological processes with refined resolution. 

10:00

Quantitative Analysis of Single-Cell Transcriptomes
Sten Linnarsson, Assistant Professor, Karolinska Institutet, Sweden

Using molecular labels to nearly eliminate PCR amplification bias and obtain absolute molecule counts by sequencing will be discussed.

10:30

Coffee Break and Networking in the Exhibition Hall

11:15

Taking Expression Profiling to New Dimensions
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

This presentation will describe single cell expression profiling of astrocytes responding to brain damage in mouse model.

11:45

Single-cell Gene Expression During Mouse Early Development
Rickard Sandberg, Scientist/Principal Investigator/Researcher, Karolinska Institutet, Sweden

In this talk I will present our current applications of Smart-Seq to mouse embryonic development. I will also stress how useful this protocol will be for addressing fundamental biological problems requiring genome-wide transcriptome profiling in individual cells.

12:15

Lunch Break and Networking in Exhibition Hall

13:30

Poster Viewing Session


Microfluidics, Microarrays and Chips

14:15

Catch it and Batch it: Picoliter Cultivation at the Single Cell Level
Dietrich Kohlheyer, Group Leader, Forschungszentrum Juelich GmbH, Germany

The talk covers the fabrication and successful utilization of microfluidic devices for the analysis of industrially relevant microbial strains. Micrometre sized chambers are used to capture single mother cells allowing the spatiotemporal analysis of isogenic microcolonies by automated time lapse microscopy. Genetically encoded fluorescence reporters give direct information on e.g. stress response and productivity.  

14:45

Probing Single Stem Cell Behavior in Microarrayed Artificial Niches
Matthias Lutolf, Head, Ecole Polytechnique Federale de Lausanne, Switzerland

This presentation will discuss novel microengineered in vitro platforms to decipher the complex crosstalk between stem cells and their microenvironment at single cell level and in high-throughput. 

15:15

Coffee Break and Networking in the Exhibition Hall

16:00

Natural Killer Cell Immune Surveillance Studied at the Single Cell Level
Bjorn Onfelt, Associate Professor, Karolinska Institute, Sweden

Each individual has a heterogeneous pool of natural killer (NK) cells consisting of cells with varying ability to kill virus-infected or tumor cells. Here, this heterogeneity is dissected by studying NK cell migration dynamics and cytotoxic response at the single cell level. 

16:30

How Cells Recognize and Remodel Their Micro-niches
Viola Vogel, Professor, ETH Zurich, Switzerland

Cells sense and remodel their environment by applying by pulling on their substrates.  As they apply tensile forces, all proteins that are part of force-bearing junctions get mechanically stretched.  We are particularly interested in deciphering how proteins can act as mechano-chemical signaling switches and thereby enable mechanotransduction processes.

17:00

Emerging Opportunities in Single Cell Analysis [SCA]—Qualitative and Quantitative Market Analysis and Competitive Environment
Enal Razvi, Managing Director, Select Biosciences Inc, United States of America

In this presentation, Select Biosciences will frame the various market segments in the broader SCA space, and provide a snapshot of our market analysis addressing the structure of these segments.  CTC analysis is one segment where emphasis is placed as this segment is positioned at the crossroads of research and clinical application. 

17:45

Round Table Discussions in the Exhibition Hall

Wednesday, 6 March 2013


Label-Free Routes

09:00

Attila TarnokKeynote Presentation

Label Free Analysis of Individual Cells Physiology by Spectral Impedance Flow Cytometry
Attila Tarnok, Research Director, University of Leipzig, Germany

This presentation will discuss the high sensitivity of AC impedance measurements and its suitability for label-free monitoring and quality control.

10:00

Raman Spectroscopic Characterization of Single Cells in a Biomedical Context
Ute Neugebauer, Principal Investigator, Jena University , Germany

The potential of Raman spectroscopy as a label-free and non-destructive vibrational spectroscopic method is demonstrated with a special focus on leukocytes. Furthermore, possible combinations of Raman spectroscopy with microfluidics or patch clamp are presented.

10:30

Coffee Break and Networking in the Exhibition Hall


Therapeutic Discovery

11:15

Are We There Yet? Stem Cells for Use Today as In Vitro Models and Tomorrow as Regenerative Therapies
John Hunt, Professor, University of Liverpool, United Kingdom

The pluripotent properties of stem cells provides a significant opportunity for their widespread application in in vitro modelling and experimentation once genotypically defined and phenotypically homogeneous populations can be reproducibly prepared.

11:45

Understanding Cell Lineage Patterns and Emergent Properties of Tumour Drug Resistance
Rachel Errington, Senior Research Scientist, Cardiff University, United Kingdom

A systems cytometry approach has been implemented to enable the transformation of timelapse sequences. Thus we determine the contribution of ‘informative single cells’ underpinning drug resistance of a perturbed tumour systems. We have dissected the operation of asymmetric events and inter-relationships of cells and thus revealed the strategies for lineage expansion under stress.

12:15

Lunch Break and Networking in Exhibition Hall

13:30

Poster Viewing Session


Signalling and Screening

14:15

Apoptosis Signalling in Tissue Homeostasis In-vivo
Sophie Vriz, Head of Laboratory, College de France , France

Combination of fluorescent physiological sensors and photocontrol of protein activity in living animals to understand tissue homeostasis at the single cell level.

14:45

Optical Nanosensors for Metabolite Monitoring in Microbial Biotechnology
Lothar Eggeling, Group Leader, Forschungszentrum Juelich Gmbh, Germany

Micro-organisms are used to produce numerous small molecules like vitamins or amino acids on a large scale exceeding alone in the case of L-lysine 1.500.000 tons annually. In all cases still a tedious strain development is required. The new methodology described in the talk enables for the first time to screen directly at the single cell level for improved producers, thus expediting strain development enormously.

15:15

Coffee Break and Networking in the Exhibition Hall

15:45

Integration of Structural and Functional Studies From Super-resolution to High Throughput
Heinrich Leonhardt, Professor, Ludwig Maximilians University Munich, Germany

An integrated approach to study structure and function in single cells is presented. This includes detection of unlabeled factors (like e.g. HIV capsid proteins), super-resolution microscopy with nanobodies and dynamic visualization of protein interactions and drug action in living cells.

16:15

Close of Conference