Tuesday, 15 March 2016

08:00

Registration


Day One Session
Session Chair: Klaus Drese, Scientific Director of the Institute, Fraunhofer ICT-IMM, Germany

09:00

Single Molecule Spectroelectrochemistry of Redox Enzymes and Reactive Oxygen Species in Nano-Optofluidic Structures
Paul Bohn, Arthur J. Schmitt Professor of Chemical and Biomolecular Engineering and Professor of Chemistry and Biochemistry, University of Notre Dame, United States of America

The evolution of lab-on-a-chip devices will likely exploit the unique properties of nanoscale structures. We combine nanoconfined double-ring electrodes, supporting high efficiency redox cycling, with zero-mode waveguides to yield a new kind LoC device.

09:30

Ulf LandegrenKeynote Presentation

Tools to Study Single Cells and Single Molecules
Ulf Landegren, Principal Investigator, University of Uppsala, Sweden

Single cell protein analyses provide important insights in cellular heterogeneity. Proximity ligation and extension technologies can offer the required sensitivity and specificity for such analyses, allowing detection of even single protein molecules in situ or in solution phase.

10:30

Droplet Digital PCR: Applications in Diagnostic and Research Virology
Keith Jerome, Professor, University of Washington, United States of America

Digital PCR (dPCR) is still mainly used in research settings, but the characteristics of dPCR also make it attractive in the clinical laboratory. In this talk we will discuss applications of dPCR in the diagnostic virology laboratory, including ultraprecise cytomegalovirus monitoring, diagnosis of chromosomally integrated human herpesvirus 6, analysis of inhibition-prone samples, and interrogation of latent HIV reservoirs.

11:00

Coffee and Networking in Exhibition Hall

11:30

Single Molecule Detection with Plasmonic Structures
Steve Blair, Professor, University of Utah, United States of America

12:15

Advances in Single Molecule Biosensing
Frank Vollmer, Principal Investigator, Max Planck Institute for the Science of Light, Germany

13:00

Lunch & Networking in Exhibition Hall

14:30

Yole DéveloppementTechnology Spotlight:
Microfluidics for Single-Cell Sample Preparation: Market & Technology Analysis
Sébastien Clerc, Market & Technology Analyst, Yole Développement

This presentation will first provide an overview of single-cell sample preparation challenges and benefits of microfluidics based solutions. Then, an overview of existing solutions along with players mapping will be presented. Finally, growth scenario will be envisaged based on the potential applications.

15:00

Menno PrinsKeynote Presentation

Biosensing Based on Particles and Single-molecule Methods
Menno Prins, Professor, Eindhoven University of Technology, Netherlands

16:00

Coffee and Networking in Exhibition Hall

16:30

Single Cell Isolation, Culturing, And Detection For Single Exosome Secretion
Yu-Hwa Lo, Professor, University of California San Diego, United States of America

The talk discusses a single cell assay that enables time lapse studies of single cell behaviors and single cell secretions under perturbations such as drugs, pH changes, transforming growth factors, etc.

17:00

Drinks Reception

Wednesday, 16 March 2016


Day Two Morning Session
Session Chair: Anders Stahlberg, Principle Investigator, University Of Gothenburg, Sweden

09:00

Getting Single Cells out of High Volume Liquid Biopsy
Klaus Drese, Scientific Director of the Institute, Fraunhofer ICT-IMM, Germany

Circulating Tumor Cells (CTCs) have high relevance for future cancer diagnostics. We present a fully automated process to isolate CTCs starting out from 7.5 mL blood. The process combines large volume liquid handling and a microfluidic cartridge.

09:30

Chwee Teck LimKeynote Presentation

Going Beyond Averaging: Single Cell Analysis to Probe the Heterogeneity of Circulating Tumor Cells
Chwee Teck Lim, Provost's Chair Professor, Principal Investigator, Mechanobiology Institute, National University of Singapore, Singapore

Tumor heterogeneity is currently a major hindrance to cancer diagnosis and treatment. Here, microfluidic technology is used to probe the molecular heterogenity of single circulating tumor cells so as to obtain patient derived information for the personalized treatment of cancer patients.

10:30

High Throughput Cell Factory Screening in Picoliter Droplets
Håkan Jönsson, Assistant Professor, KTH Royal Institute of Technology, Science for Life Laboratory, Sweden

Cell factories are the producer cells at the heart of biosustainable industry, producing biofuels, chemicals or biopharmaceuticals. Using droplet microfluidics we screening of single cells and clones at rates of up to a million cells per hour to select improved cell factories for bioindustry.

11:00

Coffee and Networking in Exhibition Hall

11:30

Taking Single Cell Profiling to New Dimensions
Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

In the first part of my talk I will describe high throughput single cell profiling with focus on the experimental workflow and analysis of data, exemplifying with the profiling of individual astrocytes from mouse brains reflecting response to trauma. I will also present multiway studies, where expression of many genes in different types of samples/cells are measured over time and how most valuable information about the molecular processes involved and expression pathways can be extracted with powerful multivariate tools such as dynamic PCA available in GenEx. Finally, I will present qPCR tomography to measure intracellular mRNA and microRNA profiles in individual cells, providing new information about the mechanism underlying asymmetric cell division.

12:15

Sample Preparation and Enrichment for Single Cell Processing and Analysis
Abraham Lee, William J. Link Professor and Chair, University of California-Irvine, United States of America

This talk will discuss some recent developments in my to process biological samples, sort and enrich for rare cells, and automate for single cell analysis.

13:00

Lunch & Networking in Exhibition Hall


Day Two Afternoon Session
Session Chair: Mikael Kubista, Professor/Founder, TATAA Biocenter AB, Sweden

14:45

Breast Cancer Stem Cell Heterogeneity and Ultasensitive Mutation Detection using Sequencing
Anders Stahlberg, Principle Investigator, University Of Gothenburg, Sweden

Defining cancer stem cell heterogeneity in breast cancer at single-cell. Error-free sequencing using SiMSen-Seq allows ultrasensitive mutation detection in liquid biopsies will be discussed. 

15:15

Single cell transcriptomics: Identifying transition states in the progression to autoimmune disease
Anne Eugster, Head of CRTD Single Cell Unit, Technical University of Dresden, Germany

15:45

Coffee and Networking in Exhibition Hall

16:15

Localization of Biomolecules and Asymmetric Cell Division During Development
Radek Sindelka, Group Leader, Institute Of Biotechnology AS CR, Czech Republic

Asymmetric cell division is a key step in differentiation and development regulation. I will present asymmetric localization of biomolecules during early determination of body axes.

16:45

Digital Microfluidics For Single-Molecule Detection And Single Cell-Analysis
Elena Perez Ruiz, Senior Scientist, KU Leuven, Netherlands

Digital microfluidics emerged recently as a promising liquid handling technology for a wide variety of applications. In this presentation its possibilities for single-molecule and single-cell detection and analysis are going to be summarized.

17:15

Close of Conference