Niek Dekker,
Principal Scientist, Discovery Sciences,
AstraZeneca
Trained as a biochemist with a PhD in enzymology and postdoctoral experience on molecular biology and structural biology. Expertise on structure-function relationships for enzymes embedded in the biological membrane. Working in drug discovery at AstraZeneca since 2000 in various managerial and project roles. Currently associate director biochemical assay development delivering assays for drug screening, and leading the activities around extracellular vesicles for drug delivery at AstraZeneca. Co-authored more than 70 peer-reviewed publications.
Engineered Extracellular Vesicles For CRISPR Gene Editing
Wednesday, 27 September 2017 at 11:30
Add to Calendar ▼2017-09-27 11:30:002017-09-27 12:30:00Europe/LondonEngineered Extracellular Vesicles For CRISPR Gene EditingExtracellular Vesicles 2017 in Cripps Court, Magdalene College, Cambridge, UKCripps Court, Magdalene College, Cambridge, UKSELECTBIOenquiries@selectbiosciences.com
Exosomes present an exciting opportunity as biological delivery vehicle of therapeutic cargo with excellent safety, low intrinsic immunogenicity, cell-specific tropism and biological delivery efficacy. Application of CRISPR/Cas9 requires the functional delivery of the ribonucleoprotein (RNP) complex of Cas9 and guide RNA. Engineering of the production cell lines can be used to introduce cargo into the exosomes, and examples for the introduction of fluorescent and luminescent proteins in exosomes will be disclosed. Uptake in recipient cells can be monitored using high content imaging to provide insight in kinetics and localization. Functional delivery can be analyzed using several assays for the gene editing event (e.g., Cel1 and TIDE). Use of exosomes for delivery of CRISPR/Cas9 has applications for ex vivo manipulation of primary cells (e.g., T cells) and in vivo editing of genes in diseased tissue.
Add to Calendar ▼2017-09-27 11:30:002017-09-27 12:30:00Europe/LondonEngineered Extracellular Vesicles For CRISPR Gene EditingExtracellular Vesicles 2017 in Cripps Court, Magdalene College, Cambridge, UKCripps Court, Magdalene College, Cambridge, UKSELECTBIOenquiries@selectbiosciences.com
