Jennifer Jones,
NIH Stadtman Investigator, Head of Transnational Nanobiology, Laboratory of Pathology,
Center for Cancer Research, National Cancer Institute
Dr. Jones is NIH Stadtman Investigator, Head of Transnational Nanobiology, Laboratory of Pathology at the Center for Cancer Research, National Cancer Institute, who is developing, refining, and applying advanced high-resolution flow cytometric methods to the characterization of EV subsets. She initiated/cofounded the international ISEV-ISAC-ISTH EV Flow Cytometry Working Group, to support the development of consensus best practices and standardization methods for the field. The ultimate goal of her research is to develop a new class of EV-based biomarkers (and methods for anlyzing those EVs) that will enable adaptve therapeutic strategies, where individual patient treatments are customized based on early responses to treatment.
Profiling EV Subsets and Cargo to Enable Adaptive Tumor- and Immuno-therapies
Tuesday, 26 September 2017 at 17:30
Add to Calendar ▼2017-09-26 17:30:002017-09-26 18:30:00Europe/LondonProfiling EV Subsets and Cargo to Enable Adaptive Tumor- and Immuno-therapiesExtracellular Vesicles 2017 in Cripps Court, Magdalene College, Cambridge, UKCripps Court, Magdalene College, Cambridge, UKSELECTBIOenquiries@selectbiosciences.com
Exosomes and small viruses fall below the detection limits of conventional flow cytometers, which has limited ways to identify, sort, and study, distinct subsets of EVs and other nanoparticles as single particles. In order to maximize information and material that can be obtained with high speed, high resolution flow cytometers we have developed nanoscale Fluorescence Associated Cytometric Sorting (nanoFACS). We have demonstrated nanoFACS to be capable of detecting tumor-cell derived EVs with specific tumor antigens, such as Prostate Specific Membrane Antigen (PSMA), with fluorescence and scattered light parameters, as well as being capable of sorting two distinct HIV strains (85-125 nm) to >95% purity. The development of our nanoFACS method provides a unique way to analyze and sort functional EV- and viral-subsets, while preserving vesicular structure, surface protein specificity, and RNA cargo activity.
Add to Calendar ▼2017-09-26 17:30:002017-09-26 18:30:00Europe/LondonProfiling EV Subsets and Cargo to Enable Adaptive Tumor- and Immuno-therapiesExtracellular Vesicles 2017 in Cripps Court, Magdalene College, Cambridge, UKCripps Court, Magdalene College, Cambridge, UKSELECTBIOenquiries@selectbiosciences.com
