Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine,
The University of Kansas
Prof. Steven A. Soper received his Ph.D. from the University of Kansas in 1989 followed by a Postdoctoral Fellowship at Los Alamos National Laboratory, where he worked on single-molecule detection for high speed sequencing of the human genome. In 1991, he joined the faculty at Louisiana State University (LSU) within the Department of Chemistry, where he filled the William H. Pryor Distinguished Chair of Chemistry. Prof. Soper also had a joint appointment in the Mechanical Engineering Department and Department of Biological Sciences. While at LSU, he founded the Center for BioModular Multi-Scale Systems, which has as its primary charge to develop enabling and transformative tools for making environmental and health-related measurements at unprecedented speeds with full process automation directly in the field. Some of these tools include monitoring air and food/water supplies for biopathogens, determining bacterial/viral infections of unknown strains in patients, evaluating brain damage/injury, sampling blood for rare cells, such as tumor cells, and their molecular analysis and sequencing genomes in near real time. These tools consist of microfluidic and nanofluidic devices make in polymers via micro- or nanoreplication.
Prof. Soper is currently Foundation Distinguished Professor, Director, Center of BioModular Multi-scale System for Precision Medicine at the University of Kansas. Prof. Soper also holds a joint appointment at Ulsan National Institute of Science and Technology in Ulsan, South Korea, where he is a World Class University Professor. Prof. Soper is currently the Editor of the Americas for the Analyst and on the Editorial Board for Journal of Fluorescence and Journal of Micro- and Nanosystems. Prof. Soper is currently serving as a permanent Member of the Nano study panel with the National Institutes of Health. As a result of his efforts, Prof. Soper has secured extramural funding totaling $45M and has published over 295 manuscripts (h index = 44) and is the author of ten patents. He is also the founder of a startup company, BioFluidica, which is marketing devices for the isolation and enumeration of rare cells. His list of awards includes Chemical Instrumentation by the American Chemical Society, the Benedetti-Pichler Award, Fellow of the AAAS, Fellow of Applied Spectroscopy, Fellow of the Royal Society of Chemistry, R&D 100 Award, Distinguished Masters Award at LSU and Outstanding Scientist/Engineer of Louisiana in 2001. Finally, Prof. Soper has granted 40 PhDs and 5 MS students under his direction.
Fabrication and Applications of Thermoplastic Nanochannels: Transport Behavior of Deoxynucleotide Monophosphates for Single-Molecule DNA Sequencing
Friday, 19 September 2014 at 09:30
Add to Calendar ▼2014-09-18 00:00:002014-09-18 01:00:00Europe/LondonTitle to be Confirmed.Lab-on-a-Chip, Microfluidics and Microarray World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
The major focus of this presentation will be to discuss new technologies, methodologies and fundamental knowledge in the area of single-molecule DNA sequencing using nanometer channels fabricated in thermoplastics. Nanochannels with dimensions <200 nm (width and depth) and lengths >50 µm are fabricated in polymer substrates using nanoimprint lithography (NIL) and resin stamps. The procedure basically involves using optical lithography and focused ion beam milling to make masters in silicon that consist of micro- and nanostructures. These masters are subsequently used to make resin stamps via UV-NIL. The resin stamps can then be used to make the required nanochannels in a variety of polymeric material, such as cyclic olefin copolymer, COC, and poly(methylmethacrylate), PMMA. Channels with dimensions to 15 nm (width x depth) and lengths to 100 µm have already been fabricated using this procedure. We will discuss the electrophoretic transport properties of deoxynucleotides monophosphates (dNMPs) through these channels that generate molecular-dependent flight times that can be used for their identification. The sequencing platform employs an exonuclease enzyme to produce the dNMPs from intact dsDNA molecules. Using fluorescence microscopy, we will show the single-molecule transport dynamics of dNMPs in a variety of nanochannel columns and compare results to the transport in microchannels. The electrophoretic migration behavior of the dNMPs using microchip electrophoresis was not possible unless cationic surfactants were added to the carrier electrolyte. However, due to transverse electromigation effects (TEM), different migration patterns of the dNMPs were observed in nanochannels. We will also discuss a host of surface modifications that can be invoked on the nanochannels to affect the surface charge density, topology and chemistry and the consequences of these modifications on the transport dynamics of dNMPs as well as the electroosmotic flow (EOF).
Add to Calendar ▼2014-09-18 00:00:002014-09-19 00:00:00Europe/LondonLab-on-a-Chip, Microfluidics and Microarray World CongressLab-on-a-Chip, Microfluidics and Microarray World Congress in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com