Steve Soper,
Foundation Distinguished Professor, Director, Center of BioModular Multi-Scale System for Precision Medicine,
The University of Kansas
Prof. Soper is currently a Foundation Distinguished Professor in Chemistry and Mechanical Engineering at the University of Kansas, Lawrence. Prof. Soper also holds an appointment at Ulsan National Institute of Science and Technology in Ulsan, South Korea, where he is a World Class University Professor. He is also serving as a Science Advisor for a number of major worldwide companies. Prof. Soper is currently on the Editorial Board for Scientific Reports and Journal of Micro- and Nanosystems.
As a result of his efforts, Prof. Soper has secured extramural funding totaling >$103M and has published over 265 peer-reviewed manuscripts (h index = 71) and is the author of 20 patents. He is also the founder of a startup company, BioFluidica, which is marketing devices for the isolation and enumeration of circulating tumor cells. His list of awards includes Chemical Instrumentation by the American Chemical Society, the Benedetti-Pichler Award for Microchemistry, Fellow of the AAAS, Fellow of Applied Spectroscopy, Fellow of the Royal Society of Chemistry, R&D 100 Award, Distinguished Masters Award at LSU and Outstanding Scientist/Engineer in the state of Louisiana in 2001. Finally, Prof. Soper has granted 60 PhDs and 6 MS degrees to students under his mentorship. He currently heads a group of 20 researchers.
Nanosensor Chips for the Single-Molecule Sequencing of DNA and RNA
Tuesday, 18 June 2019 at 11:15
Add to Calendar ▼2019-06-18 11:15:002019-06-18 12:15:00Europe/LondonNanosensor Chips for the Single-Molecule Sequencing of DNA and RNAPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
We are generating a single-molecule DNA/RNA sequencing platform that can
acquire sequencing information with high accuracy (>99%) at
unprecedented throughputs (106 nt/s). The technology employs a fluidic
chip populated with nanosensors that read the identity of individual
mononucleotides from their characteristic flight-time through a
2-dimensional (2D) nanochannel (~50 nm in width and depth; >10 µm in
length) and their current transient amplitudes. The nanosensors are
fabricated in a thermoplastic via nanoimprint lithography (NIL). The
mononucleotides are generated from an intact DNA fragment using a highly
processive exonuclease, which is covalently anchored to a plastic
support (500 nm in diameter) contained within a bioreactor that
sequentially feeds mononucleotides into a 2D nanochannel. The identity
of the mononucleotides is deduced from a molecular-dependent flight-time
through the 2D nanochannel that is related to the electrophoretic
mobility of that molecule. The flight time is read in a label-less
fashion by measuring current transients (i.e., resistive pulse sensing)
induced by a single mononucleotide when it travels through a
constriction possessing molecular dimensions (<10 nm in diameter) and
poised at the input/output ends of the flight tube. In this
presentation, our efforts in building these nanosensors using NIL in
thermoplastics will be discussed. We will also talk about the detection
of single molecules using NIL-produced nanopores. Also, surface
modifications of plastics for the immobilization of biologics, such as
exonucleases, will be discussed and their enzymatic performance when
surface immobilized. Finally, information on the manipulation of single
DNA molecules using nanofluidic circuits that uses nano-scale features
to shape electric fields will be presented.
Add to Calendar ▼2019-06-18 00:00:002019-06-19 00:00:00Europe/LondonPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019Point-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2019-06-18 11:15:002019-06-18 12:15:00Europe/LondonNanosensor Chips for the Single-Molecule Sequencing of DNA and RNAPoint-of-Care, Biosensors and Mobile Diagnostics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
