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SELECTBIO Conferences PCR & Next-Gen Sequencing

Leah Matzat's Biography

Leah Matzat, Senior Scientist, Bioo Scientific

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Molecular Indexing For Improved RNA-Seq Quantitation and Analysis

Wednesday, 30 April 2014 at 11:30

Add to Calendar ▼2014-04-30 11:30:002014-04-30 12:30:00Europe/LondonMolecular Indexing For Improved RNA-Seq Quantitation and Analysis

Most modern methods for NGS library prep require the use of enzyme processing, such as DNA polymerase reactions, which can introduce errors in the form of incorrect sequence and misrepresented copy number. Conventional RNA sequencing library construction involves the ligation of a population of cDNA molecules with adapters prior to amplification and sequencing. With Molecular Indexed™ libraries, each molecule is tagged with a molecular index randomly chosen from ~10,000 combinations so that any two identical molecules become distinguishable (with odds of 10,000/1), and can be independently evaluated in later data analysis. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.

Add to Calendar ▼2014-04-29 00:00:002014-04-30 00:00:00Europe/LondonPCR and Next-Gen