Emanuele Cocucci,
Instructor,
Boston Childrens Hospital and Harvard Medical School
Dr. Emanuele Cocucci serves an Instructor in Pediatrics at Harvard Medical School, in the laboratory of Dr. Tomas Kirchhausen. He received his MD title from Universita’ degli Studi di Milano (Milan, Italy) and his PhD title from Universita’ Vita-Salute San Raffaele (Milan, Italy). During his postdoctoral training Emanuele developed fluorescence based high resolution microscopy approaches which, coupled with computational tools, allow the detection and tracking of single molecules or objects in real time. Using these methodologies Emanuele elucidated the key role of phosphatidylinositol 4,5-bisphosphate and the stoichiometry of clathrin and adaptor molecules in the formation of clathrin coated structures. Emanuele intends now to dissect at the molecular level how extracellular vesicles form, are released and interact with target cells in physiological and pathological conditions, using similar high resolution microscopy techniques. The mechanisms involved in extracellular vesicle internalization and fusion within intracellular compartments is one of the major focuses of Emanuele’s work.
Live-Cell Imaging of Extracellular Vesicles with Single Molecule Sensitivity
Friday, 19 September 2014 at 09:15
Add to Calendar ▼2014-09-19 09:15:002014-09-19 10:15:00Europe/LondonLive-Cell Imaging of Extracellular Vesicles with Single Molecule SensitivityExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Extracellular vesicles (EVs) are membrane bound objects released by cells in the outer space, either upon fusion of multivesicular bodies with the plasma membrane (exosomes), or by direct outward budding of the plasma membrane (ectosomes). EVs retain proteins and nucleic acids of the donor cells and fuse within acceptor cells. The released content can influence the target cell behavior. Numerous classes of EVs exist, showing differences in size composition and origins. Therefore, a thoughtful study of the EV traffic is essential to define which specific classes are competent for influencing the target cells. State of the art spinning-disk confocal microscopy, coupled with computational tools, allows nowadays to detect few fluorescent molecules and to follow their trafficking in monitored cells in real-time. The application of these approach on EVs traffic will elucidate which endocytic routes are followed by the EVs, which EV subclasses are competent for fusing with endomembrane and in which subcellular compartment. The acquired knowledge will shed light on EV competence for transferring information among cells and the key EV properties which can be exploited to develop next generation drug delivery nanoparticles.
Add to Calendar ▼2014-09-18 00:00:002014-09-19 00:00:00Europe/LondonExosomes and Single Cell Analysis SummitExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2014-09-19 09:15:002014-09-19 10:15:00Europe/LondonLive-Cell Imaging of Extracellular Vesicles with Single Molecule SensitivityExosomes and Single Cell Analysis Summit in San Diego, California, USASan Diego, California, USASELECTBIOenquiries@selectbiosciences.com
