Yaoyu Wang,
Associate Director,
Dana-Farber Cancer Institute
Dr. Wang is Associate Director of the Center for Cancer Computational Biology at the Dana-Farber Cancer Institute. Trained as a bioinformatician, Dr Wang has extensive expertise in genomics, proteomics, virology, and bioinformatics. He has worked in the application of bioinformatics analysis on high throughput technologies to the understanding of human diseases and the development of clinical applications of the genomic information. He currently leads the operation at the Center for Cancer Computational Biology, which provides broad-based bioinformatics support to the community as well as develops novel high throughput sequencing technology.
Standardizing Extracellular Small RNASeq for Biomarker Discovery
Monday, 13 November 2017 at 15:00
Add to Calendar ▼2017-11-13 15:00:002017-11-13 16:00:00Europe/LondonStandardizing Extracellular Small RNASeq for Biomarker DiscoveryThe RNA Summit: Research, Diagnostics and Therapeutics in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
The presence and relative stability of extracellular RNAs (exRNAs) in biofluids has led to an emerging recognition of their promise as ‘liquid biopsies’ for diseases. The recent application of next-generation sequencing to discovery of exRNA biomarkers has revealed the presence of potential novel miRNAs as well as other RNA species such as tRNAs, snoRNAs, piRNAs and lncRNAs in biofluids. At the same time, the use of RNA sequencing for biofluids poses unique challenges, including low amounts of input RNAs, the presence of exRNAs in different compartments with varying degrees of vulnerability to isolation techniques, and the high abundance of specific RNA species (thereby limiting the sensitivity of detection of less abundant species). Moreover, discovery in human diseases often relies on archival biospecimens of varying age and limiting amounts of samples. In this study, we have tested RNA isolation methods to optimize profiling exRNAs by RNA sequencing in individuals without any known diseases. Our findings are consistent with other recent studies that detect microRNAs and ribosomal RNAs as the major exRNA species in plasma. Similar to other recent studies, we found that the landscape of biofluid microRNA transcriptome is dominated by several abundant microRNAs that appear to comprise conserved extracellular miRNAs. This study highlights the challenges in detecting and quantifying less abundant plasma miRNAs in health and disease using RNA sequencing platforms.
Add to Calendar ▼2017-11-13 00:00:002017-11-14 00:00:00Europe/LondonThe RNA Summit: Research, Diagnostics and TherapeuticsThe RNA Summit: Research, Diagnostics and Therapeutics in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2017-11-13 15:00:002017-11-13 16:00:00Europe/LondonStandardizing Extracellular Small RNASeq for Biomarker DiscoveryThe RNA Summit: Research, Diagnostics and Therapeutics in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
