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Purification of Circulating Cell-Free DNA from Plasma using the Automated QIAsymphony SP Instrument

Monday, 23 March 2015 at 14:00

Add to Calendar ▼2015-03-23 14:00:002015-03-23 15:00:00Europe/LondonPurification of Circulating Cell-Free DNA from Plasma using the Automated QIAsymphony SP InstrumentSELECTBIOenquiries@selectbiosciences.com

A new ccfDNA extraction protocol on the QIAsymphony® SP revealed a highly efficient recovery of total ccfDNA and fetal ccfDNA compared to the QIAamp® Circulating NA Kit.  Linearity was shown for ccfDNA yield from 2-6 ml plasma input. A high efficiency for extraction of low copy numbers of ccfDNA is given. Compatibility of eluates to manifold downstream assays was confirmed for different PCR and methylation-sensitive assays, library preps and subsequent NGS-based analysis of chromosomes.  Concluding, the novel protocol enables automated ccfDNA recovery from up to 4 ml plasma and up to 96 samples per QIAsymphony® SP run in 6 hours combined with high recovery efficiency.
The applications presented here are for research use only. Not for use in diagnostic procedures.