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SELECTBIO Conferences RNA-Seq, Single Cell Analysis & Single Molecule Analysis 2017

Sheng Zhong's Biography



Sheng Zhong, Professor, University of California-San Diego

Sheng Zhong is a professor at the Department of Bioengineering, University of California San Diego. His lab develops new technologies to map molecular networks, including RNA-RNA interactome, RNA-chromatin interactome, and protein-protein interactome. He received NSF CAREER Award, Alfred Sloan Fellowship, and NIH Pioneer Award. He serves as an Associate Editor for PLoS Computational Biology, and the contact PI for NIH 4D Nucleome organizational hub.

Sheng Zhong Image

Mapping RNA-RNA Interactome and RNA Structure in vivo

Friday, 6 October 2017 at 08:30

Add to Calendar ▼2017-10-06 08:30:002017-10-06 09:30:00Europe/LondonMapping RNA-RNA Interactome and RNA Structure in vivoRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

The pervasive transcription of our genome presents a possibility of revealing new genomic functions by investigating RNA interactions. Current methods for mapping RNA-RNA interactions have to rely on an “anchor” protein or RNA, and often require molecular perturbations. Here we present the MARIO (Mapping RNA interactome in vivo) technology to massively reveal RNA-RNA interactions from unperturbed cells. We mapped tens of thousands of endogenous RNA-RNA interactions from mouse embryonic stem cells and brain. We validated seven interactions by RNA antisense purification and one interaction using single molecule RNA-FISH. The experimentally derived RNA interactome is a scale-free network, which is not expected from currently perceived promiscuity in RNA-RNA interactions. Base pairing is observed at the interacting regions between long RNAs, including transposon transcripts, suggesting a class of regulatory sequences acting in trans. In addition, MARIO data reveal thousands of intra-molecule interactions, providing in vivo data on high-order RNA structures.


Add to Calendar ▼2017-10-05 00:00:002017-10-06 00:00:00Europe/LondonRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017RNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com