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Isolating Circulating Cell Free DNA from Plasma and Serum Using MagMAX Cell Free DNA isolation kit

Tuesday, 17 November 2015 at 11:00

Add to Calendar ▼2015-11-17 11:00:002015-11-17 12:00:00Europe/LondonIsolating Circulating Cell Free DNA from Plasma and Serum Using MagMAX Cell Free DNA isolation kitSELECTBIOenquiries@selectbiosciences.com

Cell-free DNA (cfDNA) circulating in blood and other body fluids is a sample type of broad interest in applications for non-invasive detection and monitoring of human cancer and other diseases. cfDNA in body fluids is typically rare in abundance making sample preparation very challenging. Current commercially available methods of preparation do not allow for enrichment of the smaller cfDNA fraction away from the contaminating genomic DNA which may lower sensitivity of detection of the cfDNA. And current workflows for preparing cfDNA from body fluids can be lengthy and cumbersome, especially when processing multiple samples simultaneously. We have developed an easier method to isolate cell free DNA from liquid biopsies. The MagMAX™ cell-free DNA isolation kit is a new magnetic bead-based sample preparation kit designed to enrich for cfDNA using a quick and simple workflow that can be performed manually or using automated platform. The enriched cfDNA is compatible with downstream assays like digital PCR, qPCR and next generation sequencing technologies. The MagMAX™ cell-free DNA workflow and analysis of the isolated cfDNA using various analytical methods will be presented.