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SELECTBIO Conferences Single Cell Analysis Summit

Michael Pfaffl's Biography



Michael Pfaffl, Professor, Technical University of Munich

Michael W. Pfaffl started 1986 to study ‘Agriculture - Animal Science’ and ‘Biotechnology’ at the Technical University of Munich (TUM). In 1997 he obtained his PhD in ‘Molecular Physiology’ in the field of molecular muscle and growth physiology at the Chair of Physiology. In June 2003 he completed his Venia Legendi (Dr. habil.) at the Center of Life and Food Sciences Weihenstephan with the title ‘Livestock transcriptomics -- Quantitative mRNA analytics in molecular endocrinology and mammary gland physiology’.
Early 2010 he became Professor of ‘Molecular Physiology’ at the TUM School of Life Sciences. Today he has reached the ‘Principal Investigator’ status at the Institute of Animal Physiology & Immunology and is one of the leading scientists in the field of Gene Quantification, RT-qPCR technology, RNA sequencing, and complex data analysis in mRNA & small-RNA expression profiling.

He is author of around 180 peer reviewed publications, 40 book chapters, and held more than 200 lectures worldwide. In March 2012 the Elsevier SciVerse Scopus Award 2012 was granted to Prof. Michael W. Pfaffl, whose top cited Scopus article entitled "A new mathematical model for relative quantification in real-time RT-PCR" published 2001 in Nucleic Acids Research 29(9) has been cited today more than 20,500 times.
He is coauthor of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines (2009) and coauthor of the dMIQE guidelines for digital PCR (2013).
Professor Michael W. Pfaffl has editorial involvements as Editor in ‘Methods’, Founding & Section Editor in ‘Biomolecular Detection and Quantification’ and Editor-in-Chief of the ‘Gene Quantification’ webportal (www.Gene-Quantification.info), the world biggest webpage around qPCR, dPCR and Gene Expression profiling techniques and applications. He is initiator and lead organizer of the qPCR, dPCR & NGS Gene Quantification Event series in Freising Weihenstephan, Germany since 2004 (www.eConferences.de).

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Quantification Noise in Single Cell Experiments

Tuesday, 25 September 2012 at 16:30

Add to Calendar ▼2012-09-25 16:30:002012-09-25 17:30:00Europe/LondonQuantification Noise in Single Cell ExperimentsSingle Cell Analysis Summit in San Diego, USASan Diego, USASELECTBIOenquiries@selectbiosciences.com

In quantitative single-cell studies, the critical part is the low amount of nucleic acids present and the resulting experimental variations. In addition biological data obtained from heterogeneous tissue are not reflecting the expression behaviour of every single-cell. These variations can be derived from natural biological variance or can be introduced externally. Both have negative effects on the quantification result. The aim of this study is to make quantitative single-cell studies more transparent and reliable in order to fulfil the MIQE guidelines at the single-cell level.


Add to Calendar ▼2012-09-25 00:00:002012-09-26 00:00:00Europe/LondonSingle Cell Analysis SummitSingle Cell Analysis Summit in San Diego, USASan Diego, USASELECTBIOenquiries@selectbiosciences.com